Nature Communications (Jul 2017)

Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis

  • Sayed Mohammad Ebrahim Sahraeian,
  • Marghoob Mohiyuddin,
  • Robert Sebra,
  • Hagen Tilgner,
  • Pegah T. Afshar,
  • Kin Fai Au,
  • Narges Bani Asadi,
  • Mark B. Gerstein,
  • Wing Hung Wong,
  • Michael P. Snyder,
  • Eric Schadt,
  • Hugo Y. K. Lam

DOI
https://doi.org/10.1038/s41467-017-00050-4
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 15

Abstract

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RNA-seq is widely used for transcriptome analysis. Here, the authors analyse a wide spectrum of RNA-seq workflows and present a comprehensive analysis protocol named RNACocktail as well as a computational pipeline leveraging the widely used tools for accurate RNA-seq analysis.