Generation of a human induced pluripotent stem cell (iPSC) line (JUCTCi011-A) from skin fibroblasts of a healthy Jordanian male subject

Nidaa A. Ababneh; Ban Al-Kurdi; Dema Ali; Raghda Barham; Nour Sharar; Mairvat M. Mrahleh; Bareqa Salah; Abdalla Awidi

Stem Cell Research (Oct 2020)

Generation of a human induced pluripotent stem cell (iPSC) line (JUCTCi011-A) from skin fibroblasts of a healthy Jordanian male subject

Nidaa A. Ababneh,
Ban Al-Kurdi,
Dema Ali,
Raghda Barham,
Nour Sharar,
Mairvat M. Mrahleh,
Bareqa Salah,
Abdalla Awidi

Affiliations

Nidaa A. Ababneh: Cell Therapy Center, The University of Jordan, Amman, Jordan; Corresponding author.
Ban Al-Kurdi: Cell Therapy Center, The University of Jordan, Amman, Jordan
Dema Ali: Cell Therapy Center, The University of Jordan, Amman, Jordan
Raghda Barham: Cell Therapy Center, The University of Jordan, Amman, Jordan
Nour Sharar: Cell Therapy Center, The University of Jordan, Amman, Jordan
Mairvat M. Mrahleh: Cell Therapy Center, The University of Jordan, Amman, Jordan
Bareqa Salah: General Surgery Department/Plastic & Reconstructive, Jordan University Hospital, The University of Jordan, Amman, Jordan
Abdalla Awidi: Cell Therapy Center, The University of Jordan, Amman, Jordan; Hemostasis and Thrombosis Laboratory, School of Medicine, the University of Jordan, Amman, Jordan; Department of Hematology and Oncology, Jordan University Hospital, Amman, Jordan

Journal volume & issue: Vol. 48
p. 101923

Abstract

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Human induced pluripotent stem cell line (JUCTCi011-A) was generated from skin fibroblasts obtained from a 34-year-old healthy male subject from Jordan. The generated iPSCs showed typical embryonic-like characteristics. They retained their normal karyotype similar to their parental dermal fibroblast cells, expressed pluripotency markers and showed a differentiation potential into three germ layers as demonstrated by immunostaining and flow cytometry. This generated cell line can be used in disease modeling studies, to serve as a healthy control line and to help in developing novel therapeutic strategies for patients with hereditary neuromuscular diseases.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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