Isolation of Mutants Using Gamma Rays and Studying Genetics of Afila and 3-Flowers/Pedicel Attributes in Garden Pea (Pisum sativum L.)

Abstract

Abstract The present study was undertaken to induce desirable mutations through γ-irradiation. A set of Lincoln and Azad P-1 varieties of garden pea were exposed to different treatments using 60Co gamma cell to determine LD50 which was estimated at 100 Gy. The frequency of total mutants including lethal ones increased with higher doses of γ-radiation that also resulted in high mutagenic effectiveness and efficiency with few exceptions. A wide range of chlorophyll and viable morphological mutations were obtained in M2 generations that include desirable characters viz., waxy leaves, 3-flowers /pedicel, short inter-nodal distance, fasciation, tall plants, edible pod, exerted stigma, powdery mildew resistance, afila type and variations for seed coat colour etc. in both the varieties. The inheritance for afila and 3-flowers per pedicel mutant in Azad P-1 were governed by a single recessive gene, as F2 generation segregates in a ratio of 1:3 for both the traits.

Keywords

• γ-rays
• induced mutation
• mutagen effectiveness
• Inheritance
• Pisum sativum L