eLife (Mar 2021)
An open-source device for measuring food intake and operant behavior in rodent home-cages
- Bridget A Matikainen-Ankney,
- Thomas Earnest,
- Mohamed Ali,
- Eric Casey,
- Justin G Wang,
- Amy K Sutton,
- Alex A Legaria,
- Kia M Barclay,
- Laura B Murdaugh,
- Makenzie R Norris,
- Yu-Hsuan Chang,
- Katrina P Nguyen,
- Eric Lin,
- Alex Reichenbach,
- Rachel E Clarke,
- Romana Stark,
- Sineadh M Conway,
- Filipe Carvalho,
- Ream Al-Hasani,
- Jordan G McCall,
- Meaghan C Creed,
- Victor Cazares,
- Matthew W Buczynski,
- Michael J Krashes,
- Zane B Andrews,
- Alexxai V Kravitz
Affiliations
- Bridget A Matikainen-Ankney
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States
- Thomas Earnest
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States
- Mohamed Ali
- National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, United States; Department of Bioengineering, University of Maryland, College Park, United States
- Eric Casey
- ORCiD
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States
- Justin G Wang
- Department of Neuroscience, Washington University in St. Louis, St. Louis, United States
- Amy K Sutton
- National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, United States
- Alex A Legaria
- Department of Neuroscience, Washington University in St. Louis, St. Louis, United States
- Kia M Barclay
- Department of Neuroscience, Washington University in St. Louis, St. Louis, United States
- Laura B Murdaugh
- Department of Neuroscience, Virginia Polytechnic and State University, Blacksburg, United States
- Makenzie R Norris
- Department of Neuroscience, Washington University in St. Louis, St. Louis, United States; Center for Clinical Pharmacology, University of Health Sciences and Pharmacy, St. Louis, United States
- Yu-Hsuan Chang
- Department of Neuroscience, Washington University in St. Louis, St. Louis, United States
- Katrina P Nguyen
- National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, United States
- Eric Lin
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States
- Alex Reichenbach
- Department of Physiology, Monash University, Clayton, Australia
- Rachel E Clarke
- Department of Physiology, Monash University, Clayton, Australia
- Romana Stark
- Department of Physiology, Monash University, Clayton, Australia
- Sineadh M Conway
- Center for Clinical Pharmacology, University of Health Sciences and Pharmacy, St. Louis, United States; Department of Anesthesiology, Washington University in St. Louis, St. Louis, United States
- Filipe Carvalho
- Open Ephys Production Site, Lisbon, Portugal
- Ream Al-Hasani
- ORCiD
- Center for Clinical Pharmacology, University of Health Sciences and Pharmacy, St. Louis, United States; Department of Anesthesiology, Washington University in St. Louis, St. Louis, United States
- Jordan G McCall
- ORCiD
- Center for Clinical Pharmacology, University of Health Sciences and Pharmacy, St. Louis, United States; Department of Anesthesiology, Washington University in St. Louis, St. Louis, United States
- Meaghan C Creed
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States; Department of Neuroscience, Washington University in St. Louis, St. Louis, United States; Department of Anesthesiology, Washington University in St. Louis, St. Louis, United States
- Victor Cazares
- Department of Psychology, Williams College, Williamstown, United States
- Matthew W Buczynski
- Department of Neuroscience, Virginia Polytechnic and State University, Blacksburg, United States
- Michael J Krashes
- ORCiD
- National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, United States
- Zane B Andrews
- ORCiD
- Department of Physiology, Monash University, Clayton, Australia
- Alexxai V Kravitz
- ORCiD
- Department of Psychiatry, Washington University in St. Louis, St. Louis, United States; Department of Neuroscience, Washington University in St. Louis, St. Louis, United States; Department of Anesthesiology, Washington University in St. Louis, St. Louis, United States
- DOI
- https://doi.org/10.7554/eLife.66173
- Journal volume & issue
-
Vol. 10
Abstract
Feeding is critical for survival, and disruption in the mechanisms that govern food intake underlies disorders such as obesity and anorexia nervosa. It is important to understand both food intake and food motivation to reveal mechanisms underlying feeding disorders. Operant behavioral testing can be used to measure the motivational component to feeding, but most food intake monitoring systems do not measure operant behavior. Here, we present a new solution for monitoring both food intake and motivation in rodent home-cages: the Feeding Experimentation Device version 3 (FED3). FED3 measures food intake and operant behavior in rodent home-cages, enabling longitudinal studies of feeding behavior with minimal experimenter intervention. It has a programmable output for synchronizing behavior with optogenetic stimulation or neural recordings. Finally, FED3 design files are open-source and freely available, allowing researchers to modify FED3 to suit their needs.
Keywords
