High-sensitive and disposable myocardial infarction biomarker immunosensor with optofluidic microtubule lasing

Abstract

The early diagnosis of myocardial infarction can significantly improve the survival rate in emergency treatment, which is mainly implemented by the immunoassay for myocardial infarction biomarkers such as cardiac troponins in blood. In this work, a disposable optofluidic microtubule whispering gallery mode (WGM) immunosensor for label-free cardiac troponin I-C (cTnI-C) complex detection has been proposed and demonstrated with active interrogation enhancement. The disposable microtubule is simply fabricated by a silica capillary with pressurized tapering technology for thin-wall, and the cTnI antibodies are immobilized on the inner wall surface of the microtubule through the self-adherent polydopamine substrate. By configuring the two coupling microfibers, the double-fiber-coupled microtubule cavity can serve as a tunable filter for the mutual-coupled polarimetric fiber ring laser (FRL), whose output laser wavelength is determined by the cTnI-C concentration in the optofluidic microtubule with inherent microfluidic channel. Due to the cyclic-cumulative gain of the FRL, the characteristic resonant peak of optical sensing signal is enhanced in the spectral width compression and the optical signal-to-noise ratio improvement, and therefore the optical immunosensor for cTnI-C can be achieved by tracking the output laser wavelength of the FRL conveniently. The dynamic binding and unbinding process of cTnI-C antigen–antibody is illustrated by monitoring the lasing peak wavelength continuously. Our all-fiber immunosensor demonstrated here has the advantages of fast label-free detection, real-time monitor, high sensitivity and disposable sensing element, which can be an innovative detecting tool in early diagnosis of myocardial infarction.

Keywords

• biomarker
• cardiac troponin
• immunosensor
• optofluidic
• whispering gallery mode