Application of Laser-Induced Breakdown Spectroscopy Combined with Chemometrics for Identification of Penicillin Manufacturers

Abstract

Due to the differences in raw materials and production processes, the quality of the same type of drug produced by different manufacturers is different. In drug supervision, determining the manufacturer can help to trace drug quality issues. In this study, a method for the quick identification of drug manufacturers based on laser-induced breakdown spectroscopy (LIBS) was proposed for the first time. We obtained the LIBS spectra from 12 samples of three types of penicillin (phenoxymethylpenicillin potassium tablets, amoxicillin capsules, and amoxicillin and clavulanate potassium tablets) produced by 10 manufacturers. The LIBS characteristic lines of the three types of penicillin were ranked by importance based on the decrease in the Gini index of random forest (RF). Three classifiers—the linear discriminant analysis (LDA), support vector machine (SVM) and artificial neural network (ANN)—were used to identify the different manufacturers of the three types of penicillin. RF-ANN provided the best classification result and an accuracy of 100% in penicillin manufacturer identification. The results show that LIBS combined with chemometrics could be used in the identification of penicillin manufacturers, and this method has application potential in drug quality supervision.

Keywords

• laser-induced breakdown spectroscopy
• qualitative analysis
• chemometric methods