Nature Communications (Jun 2016)
Long-read sequencing and de novo assembly of a Chinese genome
- Lingling Shi,
- Yunfei Guo,
- Chengliang Dong,
- John Huddleston,
- Hui Yang,
- Xiaolu Han,
- Aisi Fu,
- Quan Li,
- Na Li,
- Siyi Gong,
- Katherine E. Lintner,
- Qiong Ding,
- Zou Wang,
- Jiang Hu,
- Depeng Wang,
- Feng Wang,
- Lin Wang,
- Gholson J. Lyon,
- Yongtao Guan,
- Yufeng Shen,
- Oleg V. Evgrafov,
- James A. Knowles,
- Francoise Thibaud-Nissen,
- Valerie Schneider,
- Chack-Yung Yu,
- Libing Zhou,
- Evan E. Eichler,
- Kwok-Fai So,
- Kai Wang
Affiliations
- Lingling Shi
- Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University
- Yunfei Guo
- Zilkha Neurogenetic Institute, University of Southern California
- Chengliang Dong
- Zilkha Neurogenetic Institute, University of Southern California
- John Huddleston
- Department of Genome Sciences, Howard Hughes Medical Institute, University of Washington
- Hui Yang
- Zilkha Neurogenetic Institute, University of Southern California
- Xiaolu Han
- Genetic, Molecular, and Cellular Biology Program, Keck School of Medicine, University of Southern California
- Aisi Fu
- Wuhan Institute of Biotechnology
- Quan Li
- Zilkha Neurogenetic Institute, University of Southern California
- Na Li
- Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University
- Siyi Gong
- Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University
- Katherine E. Lintner
- Department of Pediatrics, The Ohio State University, and The Research Institute at Nationwide Children's Hospital
- Qiong Ding
- Wuhan Institute of Biotechnology
- Zou Wang
- Wuhan Institute of Biotechnology
- Jiang Hu
- Nextomics Biosciences
- Depeng Wang
- Nextomics Biosciences
- Feng Wang
- School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology
- Lin Wang
- Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Huazhong University of Science and Technology
- Gholson J. Lyon
- Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory
- Yongtao Guan
- Department of Pediatrics, Department of Molecular and Human Genetics, USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine
- Yufeng Shen
- Departments of Systems Biology and Biomedical Informatics, Columbia University
- Oleg V. Evgrafov
- Zilkha Neurogenetic Institute, University of Southern California
- James A. Knowles
- Zilkha Neurogenetic Institute, University of Southern California
- Francoise Thibaud-Nissen
- National Center for Biotechnology Information, U.S. National Library of Medicine
- Valerie Schneider
- National Center for Biotechnology Information, U.S. National Library of Medicine
- Chack-Yung Yu
- Department of Pediatrics, The Ohio State University, and The Research Institute at Nationwide Children's Hospital
- Libing Zhou
- Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University
- Evan E. Eichler
- Department of Genome Sciences, Howard Hughes Medical Institute, University of Washington
- Kwok-Fai So
- Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan University
- Kai Wang
- Zilkha Neurogenetic Institute, University of Southern California
- DOI
- https://doi.org/10.1038/ncomms12065
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 10
Abstract
Short-read sequencing has inherent limitations in the characterisation of long repeat elements. Shi and Guo et al.combine single-molecule real-time sequencing and IrysChip to construct a Chinese reference genome that fills many gaps in the reference genome, and identify novel spliced genes.
