Effects of <i>OsRCA</i> Overexpression on Rubisco Activation State and Photosynthesis in Maize
Yujiao Feng,
Hao Wu,
Huanhuan Liu,
Yonghui He,
Zhitong Yin
Affiliations
Yujiao Feng
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri–Product Safety of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, China
Hao Wu
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri–Product Safety of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, China
Huanhuan Liu
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri–Product Safety of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, China
Yonghui He
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri–Product Safety of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, China
Zhitong Yin
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri–Product Safety of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, China
Ribulose–1,5–bisphosphate carboxylase/oxygenase (Rubisco) is the rate–limiting enzyme for photosynthesis. Rubisco activase (RCA) can regulate the Rubisco activation state, influencing Rubisco activity and photosynthetic rate. We obtained transgenic maize plants that overproduced rice RCA (OsRCAOE) and evaluated photosynthesis in these plants by measuring gas exchange, energy conversion efficiencies in photosystem (PS) I and PSII, and Rubisco activity and activation state. The OsRCAOE lines showed significantly higher initial Rubisco activity and activation state, net photosynthetic rate, and PSII photochemical quantum yield than wild–type plants. These results suggest that OsRCA overexpression can promote maize photosynthesis by increasing the Rubisco activation state.
