A 65-Day Fumonisin B Exposure at High Dietary Levels Has Negligible Effects on the Testicular and Spermatological Parameters of Adult Rabbit Bucks
András Szabó,
Szabolcs Nagy,
Omeralfaroug Ali,
Zsolt Gerencsér,
Miklós Mézes,
Krisztián Milán Balogh,
Tibor Bartók,
Levente Horváth,
Aziz Mouhanna,
Melinda Kovács
Affiliations
András Szabó
MTA-KE-SZIE Mycotoxins in the Food Chain Research Group, Department of Physiology and Animal Health, Institute of Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. u. 40., 7400 Kaposvár, Hungary
Szabolcs Nagy
Department of Precision Livestock Farming and Animal Biotechnics, Institute of Animal Sciences, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, Deák F. u. 16., 8360 Keszthely, Hungary
Omeralfaroug Ali
Department of Physiology and Animal Health, Institute of Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. u. 40., 7400 Kaposvár, Hungary
Zsolt Gerencsér
Department of Animal Breeding, Institute of Animal Sciences, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. u. 40., 7400 Kaposvár, Hungary
Miklós Mézes
Department of Feed Toxicology, Institute of Physiology and Nutrition, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, Páter K. u. 1., 2053 Gödöllő, Hungary
Krisztián Milán Balogh
Department of Feed Toxicology, Institute of Physiology and Nutrition, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, Páter K. u. 1., 2053 Gödöllő, Hungary
Tibor Bartók
Fumizol Ltd., Kisfaludy u. 6/b, 6725 Szeged, Hungary
Levente Horváth
Fumizol Ltd., Kisfaludy u. 6/b, 6725 Szeged, Hungary
Aziz Mouhanna
Department of Physiology and Animal Health, Institute of Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. u. 40., 7400 Kaposvár, Hungary
Melinda Kovács
MTA-KE-SZIE Mycotoxins in the Food Chain Research Group, Department of Physiology and Animal Health, Institute of Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Guba S. u. 40., 7400 Kaposvár, Hungary
A 65-day study was undertaken to test the effects of two doses (10 and 20 mg/kg) of dietary fumonisin Bs (FB) on the rabbit male reproduction system. Body and testicular weight was not affected by the intoxication, neither the fatty acid composition of the testicular total phospholipids; the testis histological analysis failed to reveal any toxic effect. The FBs increased the testicular concentration and activity of reduced glutathione and glutathione peroxidase and decreased initial phase lipid peroxidation (conjugated dienes and trienes) in a dose dependent manner. Sperm morphology and chromatin condensation were monitored on Feulgen-stained smears. No significant differences were observed between the treatment groups and between sampling time points. The live cell ratio in the sperm (as assessed with flow cytometry) was not different among groups at any of the five sampling timepoints and was also identical within groups. Similarly, the spermatozoa membrane lipid profile was also identical in all three groups after the total intoxication period. In summary, it was demonstrated that FBs in an unrealistic and unjustified high dose still do not exert any drastic harmful effect on the leporine, male reproduction system, meanwhile slightly augmenting testicular antioxidant response.
