Next-generation sequencing yields the complete chloroplast genome of Pleione chunii, a vulnerable orchid in China

Abstract

Pleione chunii is a vulnerable epiphytic orchid with significant ornamental value. Here, we report the first complete chloroplast genome of P. chunii. The circular genome was 158,880 bp in length and consisted of a pair of inverted repeats (IR 26,465 bp), which were separated by a large single copy region (LSC 87,259 bp) and a small single copy region (SSC 18,691 bp). It contained 115 unique genes, including 87 protein-coding genes, 38 tRNAs, and eight rRNAs. The maximum-likelihood phylogenetic analysis indicated that P. chunii was sister to P. bulbocodioides and P. formosana.

Keywords

• chloroplast genome
• phylogenetic
• illumina sequencing
• pleione chunii
• orchidaceae