Stem Cell Reports (Sep 2016)
Haemopedia: An Expression Atlas of Murine Hematopoietic Cells
- Carolyn A. de Graaf,
- Jarny Choi,
- Tracey M. Baldwin,
- Jessica E. Bolden,
- Kirsten A. Fairfax,
- Aaron J. Robinson,
- Christine Biben,
- Clare Morgan,
- Kerry Ramsay,
- Ashley P. Ng,
- Maria Kauppi,
- Elizabeth A. Kruse,
- Tobias J. Sargeant,
- Nick Seidenman,
- Angela D'Amico,
- Marthe C. D'Ombrain,
- Erin C. Lucas,
- Sandra Koernig,
- Adriana Baz Morelli,
- Michael J. Wilson,
- Steven K. Dower,
- Brenda Williams,
- Shen Y. Heazlewood,
- Yifang Hu,
- Susan K. Nilsson,
- Li Wu,
- Gordon K. Smyth,
- Warren S. Alexander,
- Douglas J. Hilton
Affiliations
- Carolyn A. de Graaf
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Jarny Choi
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Tracey M. Baldwin
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Jessica E. Bolden
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Kirsten A. Fairfax
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Aaron J. Robinson
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Christine Biben
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Clare Morgan
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Kerry Ramsay
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Ashley P. Ng
- Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Maria Kauppi
- Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Elizabeth A. Kruse
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Tobias J. Sargeant
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Nick Seidenman
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Angela D'Amico
- Molecular Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Marthe C. D'Ombrain
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Erin C. Lucas
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Sandra Koernig
- CSL Limited, Parkville, VIC 3052, Australia
- Adriana Baz Morelli
- CSL Limited, Parkville, VIC 3052, Australia
- Michael J. Wilson
- CSL Limited, Parkville, VIC 3052, Australia
- Steven K. Dower
- CSL Limited, Parkville, VIC 3052, Australia
- Brenda Williams
- Biomedical Manufacturing, CSIRO Manufacturing, Clayton, VIC 3169, Australia
- Shen Y. Heazlewood
- Biomedical Manufacturing, CSIRO Manufacturing, Clayton, VIC 3169, Australia
- Yifang Hu
- Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3010, Australia
- Susan K. Nilsson
- Biomedical Manufacturing, CSIRO Manufacturing, Clayton, VIC 3169, Australia
- Li Wu
- Molecular Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Gordon K. Smyth
- Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3010, Australia
- Warren S. Alexander
- Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- Douglas J. Hilton
- Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
- DOI
- https://doi.org/10.1016/j.stemcr.2016.07.007
- Journal volume & issue
-
Vol. 7,
no. 3
pp. 571 – 582
Abstract
Hematopoiesis is a multistage process involving the differentiation of stem and progenitor cells into distinct mature cell lineages. Here we present Haemopedia, an atlas of murine gene-expression data containing 54 hematopoietic cell types, covering all the mature lineages in hematopoiesis. We include rare cell populations such as eosinophils, mast cells, basophils, and megakaryocytes, and a broad collection of progenitor and stem cells. We show that lineage branching and maturation during hematopoiesis can be reconstructed using the expression patterns of small sets of genes. We also have identified genes with enriched expression in each of the mature blood cell lineages, many of which show conserved lineage-enriched expression in human hematopoiesis. We have created an online web portal called Haemosphere to make analyses of Haemopedia and other blood cell transcriptional datasets easier. This resource provides simple tools to interrogate gene-expression-based relationships between hematopoietic cell types and genes of interest.
