Effects of ABCB1 and CYP3A5 polymorphisms on colorectal cancer susceptibility in West Algerian population: a case-control study

Abstract

Objective - The accumulation of genetic variants participates in the process involved in the risk of developing colorectal cancer (CRC). Several studies have explored the correlation between the 6986A> G polymorphisms of the CYP3A5 (CYP450) gene (rs776746) belonging to the cytochrome P450 and 3435C> T family of the adenosine triphosphate-binding cassette B1 (ABCB1) gene (rs1045642). The objective of this study is to investigate a possible association of rs776746 and rs1045642 polymorphisms with the occurrence of colorectal cancer on a population of western Algeria. Methods - The cohort consisted of 99 CRC patients and 101 controls. The CYP3A5 c.6986A> G and ABCB1 c.3435> T polymorphisms were identified by the allelic discrimination by quantitative real-time PCR technique. The results were analyzed by calculating the odd ratio (OR) with a 95% confidence interval. Results - This case-control study didn’t show any statistically significant difference in allelic and genotypic frequencies of these polymorphisms between the two groups (case and control). Conclusion - This work allowed us to conclude that there was no association between these polymorphisms and the occurrence of CRC in our population.

Keywords

• Colorectal cancer
• ABCB1
• CYP3A5
• polymorphism
• Algeria