International Journal Bioautomation (Jun 2018)

New Insights in Routine Procedure for Mathematical Evaluation of in vitro Cytotoxicity Data from Cancer Cell Lines

  • Maya M. Zaharieva,
  • Antonios Trochopoulos,
  • Lyudmila Dimitrova,
  • Martin R. Berger,
  • Hristo Najdenski,
  • Spiro Konstantinov,
  • Alexander D. Kroumov

DOI
https://doi.org/10.7546/ijba.2018.22.2.87-106
Journal volume & issue
Vol. 22, no. 2
pp. 87 – 106

Abstract

Read online

In oncopharmacology, the common procedure to evaluate median-effect concentrations (IC50) on experimental data is based on the use of well-established kinetic models representing inhibition effects of drugs on human cancer cell lines. Several widespread software programs, such as GraphPad Prism and CompuSyn offer possibilities for calculation of IC50 through the model of Chou. In recent study, we analyzed the results from those two software programs and compared them with the non-linear programming procedure written by us in the MAPLE symbolic software. The last evaluated IC50 more precisely and the correlation coefficient R value was better in all trails. We demonstrated the efficiency of non-linear programming procedures in examples of two cancer cell lines treated with three different drugs. The response surface analysis showed the potential of the applied kinetic model. As a result, we were able to define better the IC50 values and to use them in planning further experiments in human cancer cell lines related to single drug influence and drug-drug interference.

Keywords