Regulation of the endosomal SNX27-retromer by OTULIN

Aurelia Stangl; Paul R. Elliott; Adan Pinto-Fernandez; Sarah Bonham; Luke Harrison; Annalisa Schaub; Kerstin Kutzner; Kirstin Keusekotten; Paul T. Pfluger; Farid El Oualid; Benedikt M. Kessler; David Komander; Daniel Krappmann

doi:10.1038/s41467-019-12309-z

Nature Communications (Sep 2019)

Regulation of the endosomal SNX27-retromer by OTULIN

Aurelia Stangl,
Paul R. Elliott,
Adan Pinto-Fernandez,
Sarah Bonham,
Luke Harrison,
Annalisa Schaub,
Kerstin Kutzner,
Kirstin Keusekotten,
Paul T. Pfluger,
Farid El Oualid,
Benedikt M. Kessler,
David Komander,
Daniel Krappmann

Affiliations

Aurelia Stangl: Research Unit Cellular Signal Integration, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München
Paul R. Elliott: Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue
Adan Pinto-Fernandez: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford
Sarah Bonham: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford
Luke Harrison: Research Unit Neurobiology of Diabetes, Institute for Diabetes and Obesity, Helmholtz Zentrum München, German Centre for Diabetes Research (DZD)
Annalisa Schaub: Research Unit Cellular Signal Integration, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München
Kerstin Kutzner: Research Unit Cellular Signal Integration, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München
Kirstin Keusekotten: Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue
Paul T. Pfluger: Research Unit Neurobiology of Diabetes, Institute for Diabetes and Obesity, Helmholtz Zentrum München, German Centre for Diabetes Research (DZD)
Farid El Oualid: UbiQ Bio BV
Benedikt M. Kessler: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford
David Komander: Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue
Daniel Krappmann: Research Unit Cellular Signal Integration, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München

DOI: https://doi.org/10.1038/s41467-019-12309-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 18

Abstract

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OTULIN is a linear ubiquitin hydrolase that regulates ubiquitin homeostasis. Here the authors identify the adaptor of the endosomal retromer complex sorting nexin 27 (SNX27) as a binding partner of OTULIN and determine the structure of the OTULIN-SNX27 complex, which reveals a secondary interface through which OTULIN non-catalytically antagonizes SNX27 retromer assembly and cargo loading.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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