Efficacy, safety and biomarker analysis of durvalumab in patients with mismatch-repair deficient or microsatellite instability-high solid tumours
Birgit S. Geurts,
Thomas W. Battaglia,
J. Maxime van Berge Henegouwen,
Laurien J. Zeverijn,
Gijs F. de Wit,
Louisa R. Hoes,
Hanneke van der Wijngaart,
Vincent van der Noort,
Paul Roepman,
Wendy W. J. de Leng,
Anne M. L. Jansen,
Frans L. Opdam,
Maja J. A. de Jonge,
Geert A. Cirkel,
Mariette Labots,
Ann Hoeben,
Emile D. Kerver,
Adriaan D. Bins,
Frans G.L. Erdkamp,
Johan M. van Rooijen,
Danny Houtsma,
Mathijs P. Hendriks,
Jan-Willem B. de Groot,
Henk M. W. Verheul,
Hans Gelderblom,
Emile E. Voest
Affiliations
Birgit S. Geurts
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
Thomas W. Battaglia
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
J. Maxime van Berge Henegouwen
Oncode Institute
Laurien J. Zeverijn
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
Gijs F. de Wit
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
Louisa R. Hoes
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
Hanneke van der Wijngaart
Oncode Institute
Vincent van der Noort
Department of Biometrics, Netherlands Cancer Institute
Paul Roepman
Hartwig Medical Foundation
Wendy W. J. de Leng
Department of Pathology, University Medical Cancer Centre Utrecht
Anne M. L. Jansen
Department of Pathology, University Medical Cancer Centre Utrecht
Frans L. Opdam
Department of Clinical Pharmacology, the Netherlands Cancer Institute
Maja J. A. de Jonge
Department of Medical Oncology, Erasmus MC Cancer Institute
Geert A. Cirkel
Department of Medical Oncology, Meander
Mariette Labots
Department of Medical Oncology, Amsterdam University Medical Centre
Ann Hoeben
Department of Medical Oncology, Department of Internal Medicine, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Centre
Emile D. Kerver
Department of Medical Oncology, Onze Lieve Vrouwe Gasthuis
Adriaan D. Bins
Department of Medical Oncology, Amsterdam University Medical Centre
Frans G.L. Erdkamp
Department of Medical Oncology, Zuyderland Hospital
Johan M. van Rooijen
Department of Medical Oncology, Martini Hospital
Danny Houtsma
Department of Medical Oncology, Haga Hospital
Mathijs P. Hendriks
Department of Medical Oncology, Northwest Clinics
Jan-Willem B. de Groot
Department of Medical Oncology
Henk M. W. Verheul
Department of Medical Oncology, Erasmus MC Cancer Institute
Hans Gelderblom
Department of Medical Oncology, Leiden University Medical Centre
Emile E. Voest
Division of Molecular Oncology & Immunology, Netherlands Cancer Institute
Abstract Background In this study we aimed to evaluate the efficacy and safety of the PD-L1 inhibitor durvalumab across various mismatch repair deficient (dMMR) or microsatellite instability-high (MSI-H) tumours in the Drug Rediscovery Protocol (DRUP). This is a clinical study in which patients are treated with drugs outside their labeled indication, based on their tumour molecular profile. Patients and methods Patients with dMMR/MSI-H solid tumours who had exhausted all standard of care options were eligible. Patients were treated with durvalumab. The primary endpoints were clinical benefit ((CB): objective response (OR) or stable disease ≥16 weeks) and safety. Patients were enrolled using a Simon like 2-stage model, with 8 patients in stage 1, up to 24 patients in stage 2 if at least 1/8 patients had CB in stage 1. At baseline, fresh frozen biopsies were obtained for biomarker analyses. Results Twenty-six patients with 10 different cancer types were included. Two patients (2/26, 8%) were considered as non-evaluable for the primary endpoint. CB was observed in 13 patients (13/26, 50%) with an OR in 7 patients (7/26, 27%). The remaining 11 patients (11/26, 42%) had progressive disease. Median progression-free survival and median overall survival were 5 months (95% CI, 2-not reached) and 14 months (95% CI, 5-not reached), respectively. No unexpected toxicity was observed. We found a significantly higher structural variant (SV) burden in patients without CB. Additionally, we observed a significant enrichment of JAK1 frameshift mutations and a significantly lower IFN-γ expression in patients without CB. Conclusion Durvalumab was generally well-tolerated and provided durable responses in pre-treated patients with dMMR/MSI-H solid tumours. High SV burden, JAK1 frameshift mutations and low IFN-γ expression were associated with a lack of CB; this provides a rationale for larger studies to validate these findings. Trial registration Clinical trial registration: NCT02925234. First registration date: 05/10/2016.