In vivo examination of the anticoagulant effect of the Brassica oleracea methanol extract

Abstract

The anticoagulant effect of the methanol extract of Brassica oleracea var. capitata (MEB) was examined in rabbits. The animals were divided into five groups, each comprising seven animals. Three groups were administered increasing doses of MEB (200, 300, and 500 mg/kg, respectively); one group received warfarin (0.54 mg/kg); animals in the control group received saline (1 ml/day equivalent to the volume of doses applied to the treated and standard animals). Biochemical tests were performed on the 16th and 31st days of dosing. Animals that were administered MEB (500 mg MEB/kg) 30 days displayed increases of 24.07 s, 28.79 s and 4.08 s in activated partial thromboplastin (aPTT), fibrinogen (Fg) and thrombin time (TT). Compared to the control, the increase in aPTT and Fg was highly significant and the increase in TT was significant. The anticoagulant effect exhibited by MEB in rabbits may be due to inactivation or inhibition of factors affecting coagulation.

Keywords

• herbs
• anticoagulant
• rabbits
• activated partial thromboplastin time
• thrombin time and fibrinogen