Selective Detection of Folic Acid Using a Water-Stable Fluorescent CsPbBr<sub>3</sub>/Cs<sub>4</sub>PbBr<sub>6</sub> Perovskite Nanocrystal Probe
Wen-Zhi She,
Rui-Xian Zhao,
Jin-Zhou Liu,
Hai-Chi Zhang,
Rong Sheng Li,
Meng-Ting Liu,
Chuan-Hua Zhou,
Jian Ling,
Qiue Cao
Affiliations
Wen-Zhi She
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Rui-Xian Zhao
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Jin-Zhou Liu
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Hai-Chi Zhang
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Rong Sheng Li
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Meng-Ting Liu
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Chuan-Hua Zhou
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Jian Ling
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
Qiue Cao
Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), School of Chemical Science and Technology, Yunnan University, Kunming 650091, China
A water-stable cesium lead bromide (CsPbBr3/Cs4PbBr6) perovskite nanocrystal (PNC) was synthesized and studied as a fluorescence probe for the selective detection of folic acid (FA). The as-prepared PNCs emitted strong green fluorescence at 525 nm, and their structure was systematically characterized by X-ray powder diffraction, X-ray photoelectron spectroscopy, and transmission electron microscopy. The interaction between the PNCs and small biological molecules was investigated and the results indicated that the fluorescence of the PNCs could be selectively quenched by FA. The quenching rate has a linear relationship with the concentration of FA in the concentration range from 10 to 800 μM, with a correlation coefficient R2 of 0.9841, and a limit of detection (LOD, 3σ) of 1.69 μM. The mechanism of the interaction between the PNCs and FA was discussed, and the reliability of the method for real sample detection was also verified by the standard addition method. The method proposed here, using a fluorescence PNCs probe, provided a simple alternative strategy for detecting FA that will play an important role in biochemical analysis.
