A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats

Hui Chen; Hui Chen; Hui Chen; Yu Chen; Yu Chen; Huizhen Zheng; Huizhen Zheng; Huizhen Zheng; Xingwei Xiang; Xingwei Xiang; Xingwei Xiang; Lu Xu

doi:10.3389/fnut.2022.981163

Frontiers in Nutrition (Aug 2022)

A novel angiotensin-I-converting enzyme inhibitory peptide from oyster: Simulated gastro-intestinal digestion, molecular docking, inhibition kinetics and antihypertensive effects in rats

Hui Chen,
Hui Chen,
Hui Chen,
Yu Chen,
Yu Chen,
Huizhen Zheng,
Huizhen Zheng,
Huizhen Zheng,
Xingwei Xiang,
Xingwei Xiang,
Xingwei Xiang,
Lu Xu

Affiliations

Hui Chen: College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China
Hui Chen: Key Laboratory of Marine Fishery Resources Exploitment and Utilization of Zhejiang Province, Hangzhou, China
Hui Chen: Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, China
Yu Chen: Zhejiang Marine Fisheries Research Institute, Zhoushan, China
Yu Chen: Key Laboratory of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Zhoushan, China
Huizhen Zheng: College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China
Huizhen Zheng: Key Laboratory of Marine Fishery Resources Exploitment and Utilization of Zhejiang Province, Hangzhou, China
Huizhen Zheng: National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou, China
Xingwei Xiang: College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, China
Xingwei Xiang: Key Laboratory of Marine Fishery Resources Exploitment and Utilization of Zhejiang Province, Hangzhou, China
Xingwei Xiang: National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou, China
Lu Xu: Department of Animal Medicine, College of Agriculture and Forestry Science, Linyi University, Linyi, China

DOI: https://doi.org/10.3389/fnut.2022.981163
Journal volume & issue: Vol. 9

Abstract

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In this study, a novel peptide, AEYLCEAC with high angiotensin-I-converting enzyme inhibitory (ACEI) activity was screened from oyster (Crassostrea gigas) hydrolysates, which was obtained from simulated gastro-intestinal digestion. Candidate peptides were confirmed to have a higher binding to angiotensin-I-converting enzyme (ACE) than the positive drug phosphoinic tripeptide calculated by Discovery Studio, and AEYLCEAC showed the highest ACE inhibition rate in vitro with a IC50 of 4.287 mM. Lineweaver-Burk plots confirmed that the peptidic inhibitory type of ACE is competitive. The molecular docking showed that ACEI activity of the AEYLCEAC was mainly due to the hydrogen bonding interactions with the active pockets (S1 and S2) of ACE. In vivo, AEYLCEAC effectively reduced diastolic blood pressure (DBP) and Systolic blood pressure (SBP) in hypertensive rats. These results indicate that AEYLCEAC might act as a helpful ingredient in functional foods or pharmaceuticals for the prevention and treatment of hypertension.

Published in Frontiers in Nutrition

ISSN: 2296-861X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.frontiersin.org/journals/nutrition/

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