Versatile Epitope Tagging Vector for Gene Expression in Mammalian Cells

Abstract

We have constructed an epitope-tagging vector, pCMV-Tag 1, for gene expression in mammalian cells. This vector, which allows for N-terminal, C-terminal and internal tagging of the gene product of interest with the FLAG® and/or c-myc epitopes, enables researchers to rapidly and efficiently characterize gene products in vivo.