CHD6 eviction of promoter nucleosomes maintains housekeeping transcriptional program in prostate cancer

Lina Bu; Shaodong Huang; Ziyan Rao; Chenyang Wu; Bryan-Yu Sun; Yanhua Liu; Lin He; Dongyu Zhao

Molecular Therapy: Nucleic Acids (Dec 2024)

CHD6 eviction of promoter nucleosomes maintains housekeeping transcriptional program in prostate cancer

Lina Bu,
Shaodong Huang,
Ziyan Rao,
Chenyang Wu,
Bryan-Yu Sun,
Yanhua Liu,
Lin He,
Dongyu Zhao

Affiliations

Lina Bu: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China
Shaodong Huang: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China
Ziyan Rao: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China
Chenyang Wu: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China
Bryan-Yu Sun: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China
Yanhua Liu: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100191, China
Lin He: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100191, China; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100191, China
Dongyu Zhao: Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China; Corresponding author: Dongyu Zhao, Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China

Journal volume & issue: Vol. 35, no. 4
p. 102397

Abstract

Read online

CHD6, a member of the chromodomain helicase DNA-binding protein family, has been implicated in various diseases and tumors. However, its precise binding model of CHD6 on regulatory functional genes remains poorly understood. In this study, we discovered sharp peaks of CHD6, as the first member of CHD family for housekeeping process, binding only to the promoter region of genes in the C4-2 cell line. These genes, with conserved sharp CHD6 peaks across tumor cells, likely represent housekeeping genes ADNP and GOLGA5. Genes with sharp CHD6 peaks exhibit stable and low expression levels, sharing epigenetic features similar to housekeeping genes. Furthermore, this regulatory model also exists in both HEK293 cells and cardiomyocytes. Overall, the results of this study demonstrate that CHD6 binds to the promoter regions of housekeeping genes, regulating their histone modifications, chromatin structure, and gene expression.

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

About the journal

Abstract

Keywords