Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats

Abstract

Acrylamide is a vinyl monomer that is widely used for the synthesis of polyacrylamides, the treatment of drinking water, and as an additive in cosmetics. Acrylamide is also produced during the thermal processing of carbohydrate-rich foods. Although the potential toxic effects of acrylamide have been reported, few studies have evaluated biochemical parameters in blood. The present study investigated alterations of blood chemistry, hepatic function, and blood cytometry in acrylamide-treated rats. Thirty-two male Wistar rats were assigned to four experimental groups (n = 8/group): one control group received 0.3 ml of vehicle (saline solution), and the other three groups received acrylamide (25, 50, and 75 mg/kg, i.p., for 14 days). At the end of treatment, blood samples were collected to obtain serum, which was then processed using a Vitros250 device. For blood cytometry, the samples were processed in a Sysmex analyzer. The blood chemistry results showed that urea nitrogen, urea, and creatinine were elevated in the acrylamide-treated groups. Tests of hepatic function showed that total and direct bilirubins, transaminases, and alkaline phosphatase were also elevated compared with vehicle, whereas the levels of total proteins and albumin decreased. Blood cytometry showed that the levels of erythrocytes, hemoglobin, hematocrit, leukocytes, and platelets and mean cell volume decreased in the acrylamide-treated groups compared with vehicle. Overall, the present findings indicate that acrylamide causes deleterious effects on renal and hepatic physiology, producing dose-dependent alterations of blood chemistry and cytometry parameters in male Wistar rats. Keywords: Acrylamide, Blood cytometry, Blood chemistry, Hepatic function