Bio-Protocol (Jan 2012)

FACS Staining for Follicular Helper T Cells

  • Zheng Liu

DOI
https://doi.org/10.21769/BioProtoc.35
Journal volume & issue
Vol. 2, no. 2

Abstract

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Germinal center is the primary site for B cells to undergo somatic hypermutation and class switching. A recently discovered subset of T cells known as T follicular helper (TFH) cells are essential for germinal center reaction and thus have been the subjects of intensive study in the recent years. This protocol describes a reliable way to stain this population for flow analysis. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.