Frontiers in Cellular and Infection Microbiology (Feb 2021)
Rv3722c Promotes Mycobacterium tuberculosis Survival in Macrophages by Interacting With TRAF3
- Yingying Lei,
- Yingying Lei,
- Xiaojian Cao,
- Xiaojian Cao,
- Weize Xu,
- Weize Xu,
- Bing Yang,
- Bing Yang,
- Yangyang Xu,
- Yangyang Xu,
- Wei Zhou,
- Wei Zhou,
- Shuang Dong,
- Shuang Dong,
- Qijun Wu,
- Qijun Wu,
- Khaista Rahman,
- Khaista Rahman,
- Rohit Tyagi,
- Rohit Tyagi,
- Shuhong Zhao,
- Shuhong Zhao,
- Shuhong Zhao,
- Xi Chen,
- Xi Chen,
- Gang Cao,
- Gang Cao,
- Gang Cao,
- Gang Cao
Affiliations
- Yingying Lei
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Yingying Lei
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Xiaojian Cao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Xiaojian Cao
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Weize Xu
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Weize Xu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Bing Yang
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Bing Yang
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Yangyang Xu
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Yangyang Xu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Wei Zhou
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Wei Zhou
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Shuang Dong
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Shuang Dong
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Qijun Wu
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Qijun Wu
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Khaista Rahman
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Khaista Rahman
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Rohit Tyagi
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Rohit Tyagi
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Shuhong Zhao
- Key Laboratory of Agricultural Animal Genetics, Breeding, Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China
- Shuhong Zhao
- The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, China
- Shuhong Zhao
- College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Xi Chen
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Gang Cao
- State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China
- Gang Cao
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
- Gang Cao
- Bio-Medical Center, Huazhong Agricultural University, Wuhan, China
- Gang Cao
- Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China
- DOI
- https://doi.org/10.3389/fcimb.2021.627798
- Journal volume & issue
-
Vol. 11
Abstract
Mycobacterium tuberculosis (M.tb) secretes numerous proteins to interfere with host immune response for its long-term survival. As one of the top abundant M.tb secreted proteins, Rv3722c was found to be essential for bacilli growth. However, it remains elusive how this protein interferes with the host immune response and regulates M.tb survival. Here, we confirmed that Rv3722c interacted with host TRAF3 to promote M.tb replication in macrophages. Knock-down of TRAF3 attenuated the effect of Rv3722c on the intracellular M.tb survival. The interaction between Rv3722c and TRAF3 hampered MAPK and NF-κB pathways, resulting in a significant increase of IFN-β expression and decrease of IL-1β, IL-6, IL-12p40, and TNF-α expression. Our study revealed that Rv3722c interacted with TRAF3 and interrupted its downstream pathways to promote M.tb survival in macrophages. These findings facilitate further understanding of the mechanism of M.tb secreted proteins in regulating the host cell immune response and promoting its intracellular survival.
Keywords
