Screening of New <i>Dendrobium officinale</i> Strains Adapted to Karst Forest Environmental Stress Based on Electrophysiological Detection Method

Abstract

At present, the main methods of breeding Dendrobium officinale (D. officinale) include introduction and domestication, selective breeding, hybrid breeding, and mutation breeding. In the process, traditional methods of field investigation of agronomic traits are often used to select resistant varieties. Although these breeding methods are effective, they have a certain degree of subjectivity and empiricism, and the breeding cycle is long. Electrophysiological instruments were used in this experiment to test the material transport and metabolic capacity of D. officinale in a karst forest epiphytic environment and to quickly evaluate the suitability of different strains of D. officinale in a drought environment. These instruments detected the data on electrophysiological information of leaves of different strains of D. officinale under long-term drought conditions, providing immediate access to the inherent electrophysiological information of the leaves of these strains. Based on the electrophysiological parameters of D. officinale leaves as defined by the inherent electrophysiological information of plants, the water metabolism, nutrient transport, and metabolic capacity in different leaves were evaluated. The key electrophysiological indexes were verified by combining the results of chlorophyll fluorescence and chlorophyll content. The results indicate the following: (1) Parameters defined based on electrophysiological information effectively characterized the differences in intracellular water utilization, the nutrient transport status, and the metabolic capacity of different D. officinale strains. (2) The intrinsic physiological resistance, intrinsic physiological reactance, active nutrient transport capacity, and passive nutrient transport capacity were closely related to the growth status and chlorophyll function of D. officinale leaves. These electrophysiological parameters could serve as critical indicators for evaluating the drought resistance of D. officinale. (3) Under severe drought stress, strain LH1 exhibited less leaf damage, adequate water/nutrient supply, vigorous life activities, and excellent drought resistance. We found that strain LH1 demonstrates better adaptation to the arid environment of karst forest lands. The electrophysiological detection method employed in this study offers a new technique for screening wild-cultivated D. officinale resistance strains. The results indicate that the real-time online leaf electrophysiological information measured by the method in this study can characterize the energy and material metabolism of crops, greatly improving the efficiency of crop-variety selection and reducing costs. These conclusions can be used to obtain real-time information on the transmission of water and nutrients within plant cells and can provide theoretical support for studying the adaptation mechanisms of crops to adverse environments.

Keywords

• chlorophyll fluorescence
• drought stress
• electrophysiological information
• resistant strain screening