Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between

Abstract

The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche in-house projects.

Keywords

• affinity tag removal
• histidine affinity tag
• nickel chelate
• ni-nta
• protein folding
• protein purification