Immunohistochemical Evaluation of Bone Remodeling Following Compressive Force on Mandibular Condyle
Ioannis Lyros,
Ioannis A. Tsolakis,
Georgia Kotantoula,
Konstantinos Tosios,
Vilaras George,
Nikolaos Nikitakis,
Efstratios Ferdianakis,
Theodoros Lykogeorgos,
Eleni Fora,
Apostolos I. Tsolakis
Affiliations
Ioannis Lyros
Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Ioannis A. Tsolakis
Department of Orthodontics, School of Dentistry, Aristotle University of Thessaloniki, 54623 Thessaloniki, Greece
Georgia Kotantoula
Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Konstantinos Tosios
Department of Oral Medicine & Pathology and hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Vilaras George
Department of Pathology, Medical School, National &Kapodistrian University of Athens, 11527 Athens, Greece
Nikolaos Nikitakis
Department of Oral Medicine & Pathology and hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Efstratios Ferdianakis
Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Theodoros Lykogeorgos
“Hatzikosta” General Hospital of Messolonghi, 30200 Messolonghi, Greece
Eleni Fora
Department of Oral Medicine & Pathology and hospital Dentistry, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Apostolos I. Tsolakis
Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece
Excessive mandibular growth is largely affected by genetic factors and its orthodontic management is challenging. The present study utilized histophotometry and aimed to investigate immunohistochemical alterations in rat condyles following posterior mandibular displacement using a fixed intraoral functional appliance. Seventy-two male Wistar rats were divided into two equal groups, experimental and control, each consisting of three equal subgroups. The animals were sacrificed after 30, 60, and 90 days, and their condyles were isolated and examined microscopically for potential immunohistochemical changes. Statistically significant differences in RANKL appeared at days 30 and 60, while noteworthy changes regarding TRAP were evidenced at days 30 and 90. In the experimental group, RANKL decreased statistically significantly between days 60 and 90, while in the control group, the RANKL measured significantly increased at day 90 compared to days 30 and 60 alike. In the experimental group, TRAP appeared significantly decreased at day 60 vs. 30, while at day 90 vs. 60, it was found to be significantly increased. In the control group, there were no significant changes in TRAP. Posterior mandibular displacement may cause immunohistochemical changes in the rat condylar bone, and such outcomes should be considered when planning similar procedures in humans.
