Frontiers in Cell and Developmental Biology (Jun 2022)
Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus
- Joan Domingo-Reinés,
- Gonzalo Martínez-Navajas,
- Gonzalo Martínez-Navajas,
- Rosa Montes,
- Rosa Montes,
- Mar Lamolda,
- Iris Simón,
- Iris Simón,
- Julio Castaño,
- Rosa Ríos-Pelegrina,
- Rosa Ríos-Pelegrina,
- Javier Luis Lopez-Hidalgo,
- Javier Luis Lopez-Hidalgo,
- Raimundo García del Moral,
- Raimundo García del Moral,
- Juan A. Marchal,
- Juan A. Marchal,
- Juan A. Marchal,
- Juan A. Marchal,
- Pedro J. Real,
- Pedro J. Real,
- Pedro J. Real,
- Verónica Ramos-Mejía
Affiliations
- Joan Domingo-Reinés
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Gonzalo Martínez-Navajas
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Gonzalo Martínez-Navajas
- Faculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain
- Rosa Montes
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Rosa Montes
- Faculty of Sciences, Department of Cell Biology, University of Granada, Granada, Spain
- Mar Lamolda
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Iris Simón
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Iris Simón
- Faculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain
- Julio Castaño
- Advanced Cell Therapy Service, Banc de Sang I Teixits, Edifici Dr. Frederic Duran I Jordà, Barcelona, Spain
- Rosa Ríos-Pelegrina
- Unidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, Spain
- Rosa Ríos-Pelegrina
- Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain
- Javier Luis Lopez-Hidalgo
- Unidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, Spain
- Javier Luis Lopez-Hidalgo
- Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain
- Raimundo García del Moral
- Unidad de Anatomía Patológica, Hospital Universitario Clínico San Cecilio, Granada, Spain
- Raimundo García del Moral
- Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain
- Juan A. Marchal
- Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain
- Juan A. Marchal
- Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada, Spain
- Juan A. Marchal
- Faculty of Medicine, Department of Human Anatomy and Embryology, University of Granada, Granada, Spain
- Juan A. Marchal
- Excellence Research Unit “Modeling Nature” (MNat), University of Granada, Granada, Spain
- Pedro J. Real
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- Pedro J. Real
- Faculty of Science, Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain
- Pedro J. Real
- Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain
- Verónica Ramos-Mejía
- GENYO, Centre for Genomics and Oncological Research Pfizer-University of Granada-Andalusian Regional Government, Granada, Spain
- DOI
- https://doi.org/10.3389/fcell.2022.846092
- Journal volume & issue
-
Vol. 10
Abstract
Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process.
Keywords
