Cell Surface Parameters for Accessing Neutrophil Activation Level with Atomic Force Microscopy
Oksana M. Tilinova,
Vladimir Inozemtsev,
Ekaterina Sherstyukova,
Snezhanna Kandrashina,
Mikhail Pisarev,
Andrey Grechko,
Nina Vorobjeva,
Viktoria Sergunova,
Maxim E. Dokukin
Affiliations
Oksana M. Tilinova
Sarov Physics and Technology Institute, MEPhI, 607186 Sarov, Russia
Vladimir Inozemtsev
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Ekaterina Sherstyukova
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Snezhanna Kandrashina
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Mikhail Pisarev
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Andrey Grechko
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Nina Vorobjeva
Department of Immunology, Biology Faculty, Lomonosov Moscow State University, 119234 Moscow, Russia
Viktoria Sergunova
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Maxim E. Dokukin
Sarov Physics and Technology Institute, MEPhI, 607186 Sarov, Russia
In this study, we examine the topography and adhesion images of the cell surface of neutrophils during the activation process. Our analysis of cell surface parameters indicates that the most significant changes in neutrophils occur within the first 30 min of activation, suggesting that reactive oxygen species may require approximately this amount of time to activate the cells. Interestingly, we observed surface granular structure as early as 10 min after neutrophil activation when examining atomic force microscopy images. This finding aligns with the reorganization observed within the cells under confocal laser scanning microscopy. By analyzing the cell surface images of adhesion, we identified three spatial surface parameters that correlate with the activation time. This finding enables us to estimate the degree of activation by using atomic force microscopy maps of the cell surface.
