De Novo Transcriptome Analysis of the Early Hybrid Triploid Loach (<i>Misgurnus anguillicaudatus</i>) Provides Novel Insights into Fertility Mechanism
Ziyu Zhou,
Gu Shan,
Xin Li,
Wenxuan Guo,
Kexin Ma,
Yueyao Yang,
Yifan Li,
Yunbang Zhang,
He Zhou,
Xiaojuan Cao
Affiliations
Ziyu Zhou
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Gu Shan
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Xin Li
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Wenxuan Guo
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Kexin Ma
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Yueyao Yang
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Yifan Li
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Yunbang Zhang
Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
He Zhou
Key Laboratory of Mariculture, Agriculture Ministry, Dalian Ocean University, Dalian 116023, China
Xiaojuan Cao
Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
As a new freshwater aquaculture product, triploid loaches (Misgurnus anguillicaudatus) are characterized by fast growth, high-quality meat, high edibility, high resistance to disease, and sterility. In this study, a natural tetraploid loach (4n = 100) (♀) was crossed with a diploid loach (2n = 50) (♂), thus creating the hybrid triploid loach (3n = 75). The histological observations of triploid offspring and diploid controls at 4 days post-hatching (dph), 15dph, 22dph, and 50dph showed that most of the hybrid triploid loaches were abortive in the early gonad differentiation process. To explore its fertility mechanism, through transcriptome analyses of triploid offspring and diploid controls at four periods, 10 differentially expressed genes related to the early fertility mechanism were identified: amh, hormad1, rec8, h2b, plvap, zp3, h2a, nrb0b1, ddx4, and esr2. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of DEGs, two pathways were identified that are closely related to the early fertility mechanism at 50dph: the estrogen signaling pathway and steroid biosynthesis. The findings laid a foundation for further exploration of their molecular inhibition mechanism in hybrid triploid loaches.
