Nature Communications (Aug 2019)

Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions

  • Ketan D. Patel,
  • Felipe B. d’Andrea,
  • Nicole M. Gaudelli,
  • Andrew R. Buller,
  • Craig A. Townsend,
  • Andrew M. Gulick

DOI
https://doi.org/10.1038/s41467-019-11740-6
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

Read online

NocTE is a nonribosomal peptide synthetase thioesterase that completes the biosynthesis of pro-nocardicin G, the precursor for nocardicin β-lactam antibiotics. Here the authors provide mechanistic insights into NocTE by determining its crystal structures in the ligand-free form and covalently linked to a fluorophosphonate substrate mimic.