The effect of combination of regular continuous exercise and resveratrol supplementation on some regulatory and executive factors of hepatocytic apoptosis in male diabetic rats

Abstract

Background: Complex metabolic disorder with hyperglycemia is called diabetes. This study aimed to determine the effect of regular continuous exercise and resveratrol on regulatory and executive factors of hepatocytic apoptosis. Materials and Methods: In this experimental study, 60 male Wistar rats were divided into 6 groups of 10, including healthy control, diabetic, diabetic saline, resveratrol supplement, continuous exercise, and resveratrol+continuous exercise. The training program was conducted for 8 weeks and 5 days a week. Resveratrol was injected in resveratrol supplementation supplements and resveratrol+continuous exercise with a dose of 20 mg. Evaluation of apoptotic markers in the liver tissue was performed by ELISA. Results: BAX and CASPASE-3 concentrations were higher in diabetic and diabetic-saline groups (P≤0.001) .Bcl-2 concentration was also lower in the diabetic and diabetic saline groups (P≤0.001) .Mean BAX/BCL2 ratio was higher in diabetic and diabetic saline groups (P≤0.001) .Continuous exercise, resveratrol administration alone or in particular resveratrol intervention with continuous exercise, significantly decreased the mean BAX and CASPASE-3 concentrations (P≤0.001), BAX/BCL2 (P≤0.001) ratio and there was a significant increase in BCL-2 concentration (P≤0.001) in hepatocyte tissue. Conclusion: The use of non-pharmacological exercise therapy, resveratrol supplementation, and in particular the combination of these two, can be effective in reducing hepatocyte apoptosis caused by diabetes.

Keywords

• diabetes
• continuous exercise
• resveratrol
• bax
• bcl-2 and caspase-3