Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study
Raghavendra Ramalingam,
Chetna Dhand,
Chak Ming Leung,
Hariharan Ezhilarasu,
Praseetha Prasannan,
Seow Theng Ong,
Sundarapandian Subramanian,
Mohammed Kamruddin,
Rajamani Lakshminarayanan,
Seeram Ramakrishna,
Navin Kumar Verma,
Kantha Deivi Arunachalam
Affiliations
Raghavendra Ramalingam
Center for Environmental Nuclear Research, SRM Institute of Science and Technology, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
Chetna Dhand
Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore
Chak Ming Leung
Department of Biomedical Engineering, National University of Singapore, Singapore 117581, Singapore
Hariharan Ezhilarasu
Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore
Praseetha Prasannan
Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Experimental Medicine Building, 59 Nanyang Drive, Singapore 636921, Singapore
Seow Theng Ong
Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Experimental Medicine Building, 59 Nanyang Drive, Singapore 636921, Singapore
Sundarapandian Subramanian
Department of Anatomy, SRM Medical College Hospital and Research Centre, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
Mohammed Kamruddin
Materials Physics Division, Material Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamilnadu 603102, India
Rajamani Lakshminarayanan
Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore
Seeram Ramakrishna
Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore
Navin Kumar Verma
Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore
Kantha Deivi Arunachalam
Center for Environmental Nuclear Research, SRM Institute of Science and Technology, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
Electrospun fibers have emerged as promising materials in the field of biomedicine, due to their superior physical and cell supportive properties. In particular, electrospun mats are being developed for advanced wound dressing applications. Such applications require the firers to possess excellent antimicrobial properties in order to inhibit potential microbial colonization from resident and non-resident bacteria. In this study, we have developed Poly-ε-Caprolactone /gelatin hybrid composite mats loaded with natural herbal extract (Gymnema sylvestre) to prevent bacterial colonization. As-spun scaffolds exhibited good wettability and desirable mechanical properties retaining their fibrous structure after immersing them in phosphate buffered saline (pH 7.2) for up to 30 days. The initial burst release of Gymnema sylvestre prevented the colonization of bacteria as confirmed by the radial disc diffusion assay. Furthermore, the electrospun mats promoted cellular attachment, spreading and proliferation of human primary dermal fibroblasts and cultured keratinocytes, which are crucial parenchymal cell-types involved in the skin recovery process. Overall these results demonstrated the utility of Gymnema sylvestre impregnated electrospun PCL/Gelatin nanofibrous mats as an effective antimicrobial wound dressing.
