Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Abstract

ABSTRACTThe RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

Keywords

• SARS-CoV-2
• COVID 19
• rt-Real time PCR
• molecular methods
• heat treatment
• nasopharyngeal samples