Agronomía Colombiana (Dec 2011)

Morphological and anatomical analyses of the seed coats of sweet granadilla (Passiflora ligularis Juss.) seeds

  • Cárdenas Hernández Julián,
  • Miranda Lasprilla Diego,
  • Magnitskiy Stanislav,
  • Carranza Carlos

Journal volume & issue
Vol. 29, no. 3
pp. 377 – 385

Abstract

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<span style="font-size: 12pt; font-family: Arial; mso-fareast-font-family: Calibri; mso-ansi-language: ES; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"><p class="MsoNoSpacing" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-family: Calibri;"><span style="mso-ansi-language: EN-US;" lang="EN-US">The study of histology and morphology of seeds of genus <em>Passiflora </em>has been of high utility for the classification of species.<em> </em>In seeds of sweet granadilla, the histological characteristics<em> </em>and methodologies for their study are unknown. This study<em> </em>was aimed to know the tissue and morphological characteristics<em> </em>of the seed coats of seeds of sweet granadilla and to be<em> </em>able to determine its value in the differentiation of accessions.<em> </em>Five accessions collected in producing zones of the Province<em> </em>of Huila, Colombia, were analyzed. In morphological analysis,<em> </em>all accessions presented falsifoveate ornamentation and entire<em> </em>margin. The seeds presented high change in weight and size for<em> </em>every accession; there stood out the seeds of the accession PmN<em> </em>for presenting major size (7.42 mm long), weight (35.62 mg),<em> </em>homogeneity in these variables and a typical orange color. For<em> </em>the histological analysis, a protocol was adapted to realize sections<em> </em>of seed coats in parafin, by means of which one managed to<em> </em>obtain sections (7 </span>μ<span style="mso-ansi-language: EN-US;" lang="EN-US">) that in the optical microscope show clearly<em> </em>three well differentiated layers, belonging, possibly, to exotegmen<em> </em>(internal layer), mesotesta (medium layer) and exotesta<em> </em>(external layer). Every layer presented differences in the form of<em> </em>the cells, color and thickness, between the sections of the basal<em> </em>and medium parts of the seeds, but the differences between the<em> </em>analyzed accessions were not observed. The majority of seeds<em> </em>had a thickness of seed coats that varied among 235 and 475 </span>μ<span style="mso-ansi-language: EN-US;" lang="EN-US">.<em></em></span></span></p></span>

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