Mechanism of action of vinegared Cornu Cervi Degelatinatum in suppressing spleen kidney yang deficient ulcerative colitis through NCK2-JNK pathway
Tianshi Li,
Mengqi Shi,
Yan Zhao,
Zhongmei He,
Ying Zong,
Weijia Chen,
Rui Du
Affiliations
Tianshi Li
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China
Mengqi Shi
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China
Yan Zhao
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Changchun, 130118, China; Key Laboratory of Animal Production and Product Quality and Security, Ministry of Education, Ministry of National Education, Changchun, 130118, China; Corresponding author. College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, Jilin China.
Zhongmei He
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Changchun, 130118, China; Key Laboratory of Animal Production and Product Quality and Security, Ministry of Education, Ministry of National Education, Changchun, 130118, China
Ying Zong
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Changchun, 130118, China; Key Laboratory of Animal Production and Product Quality and Security, Ministry of Education, Ministry of National Education, Changchun, 130118, China
Weijia Chen
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Changchun, 130118, China; Key Laboratory of Animal Production and Product Quality and Security, Ministry of Education, Ministry of National Education, Changchun, 130118, China
Rui Du
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China; Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Changchun, 130118, China; Key Laboratory of Animal Production and Product Quality and Security, Ministry of Education, Ministry of National Education, Changchun, 130118, China; Corresponding author. College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, Jilin, China.
Background: As a traditional Chinese herbal medicine, Cornu Cervi Degelatinatum (CCD) has the effect of warming the kidney to support yang, astringing, and stopping bleeding, and is used for spleen kidney yang deficient (SKYD). This experiment was to investigate the therapeutic effects of different processes of CCD on SKYD type ulcerative colitis (UC) rats and to explore its impact on the intestinal flora of rats. Methods: ELISA was used to study the anti-inflammatory activity of Cornu Cervi Degelatinatum processed with water (WCCD) and Cornu Cervi Degelatinatum processed with vinegar (VCCD). 16SrRNA and transcriptome sequencing were used to detect the composition of rat intestinal flora and gene expression; RT-PCR and Western blot were used to verify the role of WCCD and VCCD in treating UC. Results: WCCD and VCCD have therapeutic effects on UC, could reduce tissue damage. VCCD performed better in improving Bacteroidetes/Firmicutes ratios and species evenness and abundance; performed better in increasing the quantity of lactobacillus. VCCD simultaneously inhibit the intestinal inflammatory response through NCK2, PAK4, and JNK signaling pathways. Conclusions: WCCD and VCCD play a therapeutic role in UC by regulating the proportion of different flora in the intestinal flora. VCCD regulates the intestinal flora and inflammatory response by interfering with the NCK2, PAK4 and JNK signaling pathways.
