Evaluation of stemness properties of cells derived from granulation tissue of peri‐implantitis lesions

Abstract

Abstract Objectives Peri‐implantitis (PI) is an inflammatory disease associated with peri‐implant bone loss and impaired healing potential. There is limited evidence about the presence of mesenchymal stromal cells (MSCs) and their regenerative properties within the granulation tissue (GT) of infrabony peri‐implantitis defects. The aim of the present study was to characterize the cells derived from the GT of infrabony PI lesions (peri‐implantitis derived mesenchymal stromal cells—PIMSCs). Material and Methods PIMSC cultures were established from GT harvested from PI lesions with a pocket probing depth ≥6 mm, bleeding on probing/suppuration, and radiographic evidence of an infrabony component from four systemically healthy individuals. Cultures were analyzed for embryonic (SSEA4, NANOG, SOX2, OCT4A), mesenchymal (CD90, CD73, CD105, CD146, STRO1) and hematopoietic (CD34, CD45) stem cell markers using flow cytometry. PIMSC cultures were induced for neurogenic, angiogenic and osteogenic differentiation by respective media. Cultures were analyzed for morphological changes and mineralization potential (Alizarin Red S method). Gene expression of neurogenic (NEFL, NCAM1, TUBB3, ENO2), angiogenic (VEGFR1, VEGFR2, PECAM1) and osteogenic (ALPL, BGLAP, BMP2, RUNX2) markers was determined by quantitative RT‐PCR. Results PIMSC cultures demonstrated high expression of embryonic and mesenchymal stem cell markers with inter‐individual variability. After exposure to neurogenic, angiogenic and osteogenic conditions, PIMSCs showed pronounced tri‐lineage differentiation potential, as evidenced by their morphology and expression of respective markers. High mineralization potential was observed. Conclusions This study provides evidence that MSC‐like populations reside within the GT of PI lesions and exhibit a multilineage differentiation potential. Further studies are needed to specify the biological role of these cells in the healing processes of inflamed PI tissues and to provide indications for their potential use in regenerative therapies.

Keywords

• angiogenic differentiation
• inflammatory granulation tissue
• mesenchymal stromal cells
• neurogenic differentiation
• osteogenic differentiation
• peri‐implantitis