Decellularized Umbilical Cord as a Scaffold to Support Healing of Full-Thickness Wounds
Albina A. Kondratenko,
Dmitry V. Tovpeko,
Daniil A. Volov,
Lidia I. Kalyuzhnaya,
Vladimir E. Chernov,
Ruslan I. Glushakov,
Maria Y. Sirotkina,
Dmitry A. Zemlyanoy,
Natalya B. Bildyug,
Sergey V. Chebotarev,
Elga I. Alexander-Sinclair,
Alexey V. Nashchekin,
Aleksandra D. Belova,
Alexey M. Grigoriev,
Ludmila A. Kirsanova,
Yulia B. Basok,
Victor I. Sevastianov
Affiliations
Albina A. Kondratenko
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Dmitry V. Tovpeko
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Daniil A. Volov
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Lidia I. Kalyuzhnaya
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Vladimir E. Chernov
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Ruslan I. Glushakov
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Maria Y. Sirotkina
Cellular biotechnology Centre for Cell Technology (CCT), Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia
Dmitry A. Zemlyanoy
Department of General Hygiene, St. Petersburg State Pediatric Medical University, 194100 St. Petersburg, Russia
Natalya B. Bildyug
Cellular biotechnology Centre for Cell Technology (CCT), Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia
Sergey V. Chebotarev
Research Department of Biomedical Research of the Research Center, S.M. Kirov Military Medical Academy, 194044 St. Petersburg, Russia
Elga I. Alexander-Sinclair
Cellular biotechnology Centre for Cell Technology (CCT), Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia
Alexey V. Nashchekin
Laboratory “Characterization of Materials and Structures of Solid State Electronics”, Ioffe Institute, 194021 St. Petersburg, Russia
Aleksandra D. Belova
Department for Biomedical Technologies and Tissue Engineering. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia
Alexey M. Grigoriev
Department for Biomedical Technologies and Tissue Engineering. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia
Ludmila A. Kirsanova
Department for Biomedical Technologies and Tissue Engineering. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia
Yulia B. Basok
Department for Biomedical Technologies and Tissue Engineering. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia
Victor I. Sevastianov
Department for Biomedical Technologies and Tissue Engineering. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, Russia
The umbilical cord is a material that enhances regeneration and is devoid of age-related changes in the extracellular matrix (ECM). The aim of this work was to develop a biodegradable scaffold from a decellularized human umbilical cord (UC-scaffold) to heal full-thickness wounds. Decellularization was performed with 0.05% sodium dodecyl sulfate solution. The UC-scaffold was studied using morphological analysis methods. The composition of the UC-scaffold was studied using immunoblotting and Fourier transform infrared spectroscopy. The adhesion and proliferation of mesenchymal stromal cells were investigated using the LIVE/DEAD assay. The local reaction was determined by subcutaneous implantation in mice (n = 60). A model of a full-thickness skin wound in mice (n = 64) was used to assess the biological activity of the UC-scaffold. The proposed decellularization method showed its effectiveness in the umbilical cord, as it removed cells and retained a porous structure, type I and type IV collagen, TGF-β3, VEGF, and fibronectin in the ECM. The biodegradation of the UC-scaffold in the presence of collagenase, its stability during incubation in hyaluronidase solution, and its ability to swell by 1617 ± 120% were demonstrated. Subcutaneous scaffold implantation in mice showed gradual resorption of the product in vivo without the formation of a dense connective tissue capsule. Epithelialization of the wound occurred completely in contrast to the controls. All of these data suggest a potential for the use of the UC-scaffold.
