Effects of High Dietary Carbohydrate Levels on Growth Performance, Enzyme Activities, Expression of Genes Related to Liver Glucose Metabolism, and the Intestinal Microbiota of <i>Lateolabrax maculatus</i> Juveniles
Luzhe Zheng,
Zhanzhan Wang,
Bo Zhang,
Lulu Yan,
Pengfei Wang,
Chao Zhao,
Heizhao Lin,
Lihua Qiu,
Chuanpeng Zhou
Affiliations
Luzhe Zheng
College of Fisheries and Life Sciences, Dalian Ocean University, Dalian 116023, China
Zhanzhan Wang
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
Bo Zhang
Key Laboratory of Fishery Resources Development and Utilization in South China Sea, South China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Lulu Yan
Key Laboratory of Fishery Resources Development and Utilization in South China Sea, South China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Pengfei Wang
Key Laboratory of Fishery Resources Development and Utilization in South China Sea, South China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Chao Zhao
Key Laboratory of Fishery Resources Development and Utilization in South China Sea, South China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Heizhao Lin
Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572426, China
Lihua Qiu
Key Laboratory of Fishery Resources Development and Utilization in South China Sea, South China Sea Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Chuanpeng Zhou
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
The present study was conducted to investigate the effects of high dietary carbohydrate levels on growth performance, enzyme activities, and gene expressions related to liver glucose metabolism and the intestinal microbiota of Lateolabrax maculatus juveniles. Two experimental diets with levels of carbohydrates (20% and 30%, named the NCD group and the HCD group, respectively) were designed to feed L. maculatus (initial weight 9.45 ± 0.03 g) for 56 days. The results showed that, compared with the NCD group, the condition factor (CF) was significantly elevated in the HCD group (p p p p HK) levels were significantly higher than those in the NCD group (p GK) and glycogen synthetase kinase-3 (GSK3β) genes in the HCD group was significantly increased (p PEPCK) and glycogen phosphorylase (GP) genes in the HCD group was significantly reduced (p L. maculatus, high carbohydrates led to increased condition factor and liver glycogen, lipid deposition, decreased antioxidant capacity of the liver, increased relative abundance of harmful intestinal microorganisms, and disrupted glucose metabolism.
