Applications of blood group genotyping

Abstract

Introduction: The determination of blood group polymorphism atthe genomic level facilitates the resolution of clinical problemsthat cannot be addressed by hemagglutination. They are useful to(a) determine antigen types for which currently available antibodiesare weakly reactive; (b) type patients who have been recentlytransfused; (c) identify fetuses at risk for hemolytic disease of thenewborn; and (d) to increase the reliability of repositories of antigennegative RBCs for transfusion. Objectives: This review assessedthe current applications of blood group genotyping in transfusionmedicine and hemolytic disease of the newborn. Search strategy:Blood group genotyping studies and reviews were searched ingeneral database (MEDLINE) and references were reviewed.Selection criteria: All published data and reviews were eligible forinclusion provided they reported results for molecular basis ofblood group antigens, DNA analysis for blood group polymorphisms,determination of fetal group status and applications of blood groupgenotyping in blood transfusion. Data collection: All data werecollected based on studies and reviews of blood grouppolymorphisms and their clinical applications.

Keywords

• Genotype
• Blood group antigens
• Hemaglutination
• Blood transfusion
• Erythroblastosis
• fetal
• Blood grouping and crossmatching