Derivatizing agents for spectrophotometric and spectrofluorimetric determination of pharmaceuticals: a review

Abstract

Most drug structures lack appropriate chromophores that can be examined at wavelengths other than the generic UV part of the electromagnetic spectrum. Indirect spectrophotometric/spectrofluorimetric analysis involving chemical derivatization in which molecules lacking UV-Vis absorbing moieties are transformed into derivatives with good chromophores. The article highlights the discussion of significant derivatizing agents for the derivatization of drug molecules that lack the chromophoric moiety required for spectrometric analysis. In addition, the probable reaction for colour generation is also discussed. The current developments of derivatizing agents for the spectrophotometric determination of pharmaceuticals are considered.

Keywords

• Derivatizing agents
• spectrophotometry
• pharmaceuticals
• charge transfer complex
• derivatization
• chromophore