Necdin and neuronatin genes expression among diploid parthenogenetic, IVF and in vivo derived female sexed embryos during preimplantation development in goat

Abstract

The present research work was undertaken to compare the expression of necdin (Ndn) and neuronatin (Nnat) genes among diploid parthenogenetic (DIP), female sexed in vivo and female sexed in vitro produced embryonic cell colony. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and activated by ethanol (7%) for 5 min followed by incubation with 2mM 6-dimethyl amino purine (DMAP) for 4 hrs for DIP embryos production. Embryonic cell colony were developed from 8–16 and morula stage DIP, IVF and in vivo derived embryos and these colonies were used for studying the Ndn and Nnat gene expression. There was no expression of Ndn gene in both the stages of DIP embryos, while this gene was expressed almost similarly in IVF and in vivo derived 8–16 cell stages but down regulated significantly in morula of IVF compared to in vivo derived embryonic cell colony. The Nnat gene expression was absent in in vivo derived 8–16 cell and morula stage embryos, while it was expressed in both the stages of DIP and IVF embryos. Further, it was observed that the expression of this gene was significantly lower in DIP embryos of both the stages as compared to IVF one. There was no significant difference of this gene expression between 8–16 cell and morula of DIP but morula of IVF showed significantly higher expression than 8–16 cell stage.

Keywords

• Cytochalasin B
• Diploid embryos
• Developmental gene
• Ndn
• Nnat