PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts

Ann Liza Piberger; Akhil Bowry; Richard D. W. Kelly; Alexandra K. Walker; Daniel González-Acosta; Laura J. Bailey; Aidan J. Doherty; Juan Méndez; Joanna R. Morris; Helen E. Bryant; Eva Petermann

doi:10.1038/s41467-020-19570-7

Nature Communications (Nov 2020)

PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts

Ann Liza Piberger,
Akhil Bowry,
Richard D. W. Kelly,
Alexandra K. Walker,
Daniel González-Acosta,
Laura J. Bailey,
Aidan J. Doherty,
Juan Méndez,
Joanna R. Morris,
Helen E. Bryant,
Eva Petermann

Affiliations

Ann Liza Piberger: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Akhil Bowry: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Richard D. W. Kelly: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Alexandra K. Walker: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Daniel González-Acosta: Molecular Oncology Program, Spanish National Cancer Research Centre
Laura J. Bailey: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer
Aidan J. Doherty: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer
Juan Méndez: Molecular Oncology Program, Spanish National Cancer Research Centre
Joanna R. Morris: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham
Helen E. Bryant: Department of Oncology & Metabolism, The Medical School, University of Sheffield
Eva Petermann: Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

DOI: https://doi.org/10.1038/s41467-020-19570-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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Bulky DNA adducts are important replication-blocking lesions. Here the authors reveal that homologous recombination at bulky adducts in mammalian cells involves post-replicative gap repair in a PrimPol dependent manner.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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