Small molecule carbon source promoting dairy wastewater treatment of Rhodospirillum rubrum by co-metabolism and the establishment of multivariate nonlinear equation

Abstract

Rhodospirillum rubrum water treatment technology could recycle bio-resource. However, the inability to degrade macromolecular organics limited its wide application. This paper discussed the feasibility of small molecular carbon source promoting R. rubrum directly treating dairy machining wastewater (DMW) and accumulations for single cell protein and pigment, and establishment of a mathematical model. Six small molecules promoted the degradation of macromolecules (proteins) in DMW. They promoted protease secretion and non-growth matrix (protein) decomposition in DMW through co-metabolism. Among the molecules, 550 mg/L potassium sodium tartrate was the best, protease activity and protein removal rate were increased by 100% compared with control. Then chemical oxygen demand (COD) and protein removal rates reached 80%, the single cell protein, carotenoid and bacterial chlorophyll yields were increased 2 times. Meanwhile, carbon nitrogen ratio (C/N) and food microbial ratio (F/M) were identified as the most important factors by principal component analysis. A multivariate nonlinear equation model between COD removal rate and C/N, F/M, time was established. HIGHLIGHTS R. rubrum directly treated wastewater, recycled biomass by small molecular.; Small molecules promoted protein decomposition through a co-metabolism mechanism.; Under 550 mg/L PST, contaminants removal and bioresource yields were the highest.; Multivariate nonlinear equation model on COD removal rate was established.;

Keywords

• co-metabolism
• multivariate nonlinear equation model
• r rubrum
• small molecular carbon source
• wastewater