Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Tiziano Ingegnere; Francesca Romana Mariotti; Andrea Pelosi; Concetta Quintarelli; Concetta Quintarelli; Biagio De Angelis; Nicola Tumino; Francesca Besi; Claudia Cantoni; Franco Locatelli; Paola Vacca; Lorenzo Moretta

doi:10.3389/fimmu.2019.00957

Frontiers in Immunology (Apr 2019)

Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Tiziano Ingegnere,
Francesca Romana Mariotti,
Andrea Pelosi,
Concetta Quintarelli,
Concetta Quintarelli,
Biagio De Angelis,
Nicola Tumino,
Francesca Besi,
Claudia Cantoni,
Franco Locatelli,
Paola Vacca,
Lorenzo Moretta

Affiliations

Tiziano Ingegnere: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Francesca Romana Mariotti: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Andrea Pelosi: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Concetta Quintarelli: Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy
Concetta Quintarelli: Department of “Medicina Clinica e Chirurgia”, University of Naples Federico II, Naples, Italy
Biagio De Angelis: Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy
Nicola Tumino: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Francesca Besi: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Claudia Cantoni: Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genoa and Istituto G. Gaslini, Genoa, Italy
Franco Locatelli: Department of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy
Paola Vacca: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy
Lorenzo Moretta: Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, Italy

DOI: https://doi.org/10.3389/fimmu.2019.00957
Journal volume & issue: Vol. 10

Abstract

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CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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Abstract

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