Frontiers in Plant Science (Nov 2021)
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing
- Fuquan Ce,
- Fuquan Ce,
- Fuquan Ce,
- Fuquan Ce,
- Jiaqin Mei,
- Jiaqin Mei,
- Jiaqin Mei,
- Haiyan He,
- Haiyan He,
- Haiyan He,
- Yu Zhao,
- Yu Zhao,
- Wenhui Hu,
- Wenhui Hu,
- Wenhui Hu,
- Fengqun Yu,
- Qinfei Li,
- Qinfei Li,
- Qinfei Li,
- Xuesong Ren,
- Xuesong Ren,
- Xuesong Ren,
- Jun Si,
- Jun Si,
- Jun Si,
- Hongyuan Song,
- Hongyuan Song,
- Hongyuan Song,
- Wei Qian,
- Wei Qian,
- Wei Qian
Affiliations
- Fuquan Ce
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Fuquan Ce
- College of Agronomy and Biotechnology, Southwest University, Chongqing, China
- Fuquan Ce
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Fuquan Ce
- Academy of Agricultural Sciences, Southwest University, Chongqing, China
- Jiaqin Mei
- College of Agronomy and Biotechnology, Southwest University, Chongqing, China
- Jiaqin Mei
- Academy of Agricultural Sciences, Southwest University, Chongqing, China
- Jiaqin Mei
- State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, China
- Haiyan He
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Haiyan He
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Haiyan He
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Yu Zhao
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Yu Zhao
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Wenhui Hu
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Wenhui Hu
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Wenhui Hu
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Fengqun Yu
- Agriculture and Agri-Food Canada, Saskatoon Research and Development Center, Saskatoon, SK, Canada
- Qinfei Li
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Qinfei Li
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Qinfei Li
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Xuesong Ren
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Xuesong Ren
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Xuesong Ren
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Jun Si
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Jun Si
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Jun Si
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Hongyuan Song
- College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China
- Hongyuan Song
- Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China
- Hongyuan Song
- Chongqing Key Laboratory of Olericulture, Chongqing, China
- Wei Qian
- College of Agronomy and Biotechnology, Southwest University, Chongqing, China
- Wei Qian
- Academy of Agricultural Sciences, Southwest University, Chongqing, China
- Wei Qian
- State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, China
- DOI
- https://doi.org/10.3389/fpls.2021.703520
- Journal volume & issue
-
Vol. 12
Abstract
Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.
Keywords
- Brassica oleracea
- Plasmodiophora brassicae
- clubroot
- genomic resequencing
- candidate gene
- functional marker
