Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
Núbia da Silva,
Marcos Oliveira,
Humberto Filho,
Luiz Pinheiro,
Rosana Kolb,
Odemir Martinez Bruno
Affiliations
Núbia da Silva
Institute of Mathematics and Computer Science, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13566-590, São Carlos, São Paulo, BrazilInstitute of Biotechnology, Federal University of Goiás, UFG, Av. Dr. Lamartine Pinto de Avelar, 1200, 75704-020, Catalão, Goiás, Brazil
Marcos Oliveira
Institute of Mathematics and Computer Science, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13566-590, São Carlos, São Paulo, BrazilSão Carlos Institute of Physics, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13560-970, São Carlos, São Paulo, Brazil
Humberto Filho
São Carlos Institute of Physics, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13560-970, São Carlos, São Paulo, Brazil
Luiz Pinheiro
Department of Biological Sciences, School of Sciences, Humanities and Languages, São Paulo State University, UNESP, Av. Dom Antonio, 2100, 19806-900, Assis, São Paulo, BrazilBiosciences Institute, São Paulo State University, UNESP, Av. 24A, 1515, 13506-900, Rio Claro, São Paulo, Brazil
Rosana Kolb
Department of Biological Sciences, School of Sciences, Humanities and Languages, São Paulo State University, UNESP, Av. Dom Antonio, 2100, 19806-900, Assis, São Paulo, BrazilBiosciences Institute, São Paulo State University, UNESP, Av. 24A, 1515, 13506-900, Rio Claro, São Paulo, Brazil
Odemir Martinez Bruno
Institute of Mathematics and Computer Science, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13566-590, São Carlos, São Paulo, BrazilSão Carlos Institute of Physics, University of São Paulo, USP, Av. Trabalhador são-carlense, 400, 13560-970, São Carlos, São Paulo, Brazil
The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of texture image analysis are used to extract characteristics from samples of imaged leaves and thus predict the species. Texture image analysis is a versatile and powerful technique able to extract measurements from patterns in the images. Using this technique, recent research has found a close relationship between texture and plant species (da Silva et al., 2015 and 2016). Here, we describe the procedure to extract texture features from microscopic images of leaves using Fourier (Cosgriff, 1960; Azencott, 1997; Gonzalez and Woods, 2006). It is important to highlight that other methods for texture extraction can be used as well.This protocol is split into two parts: (A) leaf epidermal dissociation and (B) automatic method for leaf epidermal image analysis.
