Nature Communications (Nov 2022)
A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1
- Yanan Zhao,
- Jiaojiao Hu,
- Shan-Shan Yang,
- Jing Zhong,
- Jianping Liu,
- Shuo Wang,
- Yuzhuo Jiao,
- Fang Jiang,
- Ruiyang Zhai,
- Bingnan Ren,
- Hua Cong,
- Yuwei Zhu,
- Fengtong Han,
- Jixian Zhang,
- Yue Xu,
- Zhiwei Huang,
- Shengnan Zhang,
- Fan Yang
Affiliations
- Yanan Zhao
- School of Life Science and Technology, Harbin Institute of Technology
- Jiaojiao Hu
- Interdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of Sciences
- Shan-Shan Yang
- State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology
- Jing Zhong
- School of Life Science and Technology, Harbin Institute of Technology
- Jianping Liu
- Interdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of Sciences
- Shuo Wang
- HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology
- Yuzhuo Jiao
- School of Life Science and Technology, Harbin Institute of Technology
- Fang Jiang
- School of Life Science and Technology, Harbin Institute of Technology
- Ruiyang Zhai
- School of Life Science and Technology, Harbin Institute of Technology
- Bingnan Ren
- School of Life Science and Technology, Harbin Institute of Technology
- Hua Cong
- School of Life Science and Technology, Harbin Institute of Technology
- Yuwei Zhu
- HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology
- Fengtong Han
- School of Life Science and Technology, Harbin Institute of Technology
- Jixian Zhang
- School of Life Science and Technology, Harbin Institute of Technology
- Yue Xu
- School of Life Science and Technology, Harbin Institute of Technology
- Zhiwei Huang
- HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology
- Shengnan Zhang
- Interdisciplinary Research Center on Biology and Chemistry, Shanghai institute of Organic Chemistry, Chinese Academy of Sciences
- Fan Yang
- School of Life Science and Technology, Harbin Institute of Technology
- DOI
- https://doi.org/10.1038/s41467-022-34551-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
Here, the authors report the discovery of a redox switch for AcrIIC1, which regulates AcrIIC1’s monomer-dimer interconversion and inhibitory activity on Cas9. By reporting the structures of different redox states of NmeAcrIIC1, they identify a pair of conserved cysteines which mediate the redox switch. The replacement of the redox-sensitive cysteines allows the generation of a more stable and robust variant under various cellular environments.
