Construction and Analysis of Subtractive cDNA Library of Muscular Tissue in Hybrid of Chicken-Quail Related to Myogenesis

Abstract

Myogenesis is a complex developmental progress in which a variety of transcription factors play essential roles in regulating myogenesis. However, the genetics programs that control myogenesis molecular mechanism are poorly understood. Therefore, meat-breeding and egg-breeding chicken were selected as male parent, Korean quails were used as female parent for hybridizing. To identify differentially expressed genes between the different hybrids, suppression subtractive hybridization (SSH) have been undertaken for generating cDNA collections of representative mRNAs specific to muscle tissue with meat-hybrid versus egg-hybrid. Following SSH, 54 clones were sequenced and analysed through BLASTX. The results showed that 26 Expressed Sequence Tags (ESTs) found no homology while the other 22 ESTs found the homology in GenBank and 4 ESTs might have correlation with myogenesis.

Keywords

• gene expression
• hybrid of chicken-quail
• muscular tissue
• myogenesis
• suppression subtractive hybridization