Molecular Diagnosis of CoccidioidesPosadasii from Aborted Placenta of Ewes by RT-PCR

Abstract

Diagnosis of diseases depends on histology using formaldehyde-fixed paraffin embedded tissue .The polymerase chain reaction (PCR)helps the pathologists to confirm the diagnosis of many disease including mycotic infections. Aborted placentas from 40 ewes were formalin fixed, paraffin embedded and sectioned. Tissue sections were stained with haematoxylin and eosine, and periodic acid Schiff (PAS) for histopathology examination. Fresh tissue parts were cultivated ,subjected to DNA extraction for the purpose of real-time PCR amplification. Histopathological examination of placental tissue revealed extensive necrotizing placentitis with the presence of spherule of CoccidioidesPosadasiiin the necrotic area. Only eight samples gave positive growth for coccidioidesPosadasii out of forty aborted placental samples. DNA amplification by real-time PCR revealed positive amplification for the eight samples which confirmed the detection of coccidiodesposadasii.

Keywords

• Coccidioides posadasii
• ewes
• necrotic placentitis
• real-time PCR