Evaluation by polymerase chain reaction assay of persistent shedding of pathogenic leptospires in the urine of dogs with leptospirosis

Abstract

Abstract Background Persistent leptospiruria in naturally infected dogs occurs despite appropriate antibiotic treatment. Hypothesis/Objectives To determine the frequency of persistent leptospiruria in naturally infected dogs and the association of persistent leptospiruria with different antibiotic treatments. Animals Thirty‐two dogs of varying age and breed diagnosed with leptospirosis via urine polymerase chain reaction assay (PCR). Methods A prospective observational study of dogs diagnosed with leptospirosis was undertaken to determine the frequency of persistent leptospiruria as determined by PCR. Clinical presentation of leptospirosis, antibiotic treatment, serum creatinine concentration, and outcome were recorded. Results Fifteen of 32 dogs had a negative urine PCR on the first submission in the study, 5 of 15 received only an aminopenicillin. The remaining 17 dogs had a negative urine PCR on the second (n = 6 dogs), third (n = 5), fourth (n = 5), and eighth (n = 1) submissions. Acute kidney injury was reported in 32/32 dogs. Two of 32 dogs developed chronic kidney disease. Conclusions and Clinical Importance Persistent leptospiruria is common despite treatment with antibiotics frequently recommended for treatment. Follow‐up urine PCR to confirm clearance of the organism is recommended in all dogs. In dogs with persistent leptospiruria, chronic kidney disease can develop after acute kidney injury.

Keywords

• antibiotic resistance
• doxycycline
• leptospirosis
• leptospiruria